The analysis of the M. perniciosa x MT interaction showed some evidences to be a case of a broken NHR by the S-biotype in tomato, but not by the C-biotype (Deganello et al., 2014 Plant Pathology; Scotton et al., 2016, Molecular Plant Pathology). The limited rate of MT penetration by basidiospores with emerging mycelia suggest a little developed pathogenic interaction between the S-biotype and MT. Molecular features of NHR will be evaluated by RNA-seq and NHR candidate gene expression analysis in the S- and C-biotype x MT interactions, together with histological observations of early penetration events. Recent reports in other pathosystems suggest that, besides effector proteins, pathogens can employ small RNAs (sRNAs) to directly manipulate the plant defense system, and, therefore, identification and expression analysis of sRNAs will be conducted during MT infection by M. perniciosa. sRNAs expression will be followed by RNA-seq during infection to investigate if this mechanism is also employed by M. perniciosa.
The opening requires an independent, creative, pro-active, researcher, with excellent ability to plan and conduct experiments, capacity to work in a team and able to write scientific articles. Candidates must have previous experience with various molecular biology and gene expression analysis approaches, solid bioinformatic and statistical knowledge, and fluency in English. Previous experience in plant pathology and/or fungi will be considered as an advantage, but it is not a requisite. Candidates must have completely their PhD recently (less than 7 years) in fields related to the project. It is expected that the individual will be capable to independently lead the project and to advise undergraduate students in activities associated with his/her activities. It is also expected that the individual collaborates in other graduate students (MSc and PhD) projects associated with the main Thematic project. The fellowship is associated with the Thematic project FAPESP 2016/10498-4, and it s planned to start by July 1st 2017 for 24 months.
Additional Salary Information: R$ 81,831.60/year (app. US$ 25,600.00/year) plus Research Contingency Funds (15% of the annual value of the fellowship).
The University of Sao Paulo (USP) is the largest research University in Brazil, responsible for around 25% of the scientific production of Brazil. It is owned by the state of Sao Paulo. A total of 60,000 undergraduate and 30,000 graduate students with 5,600 faculty. The Center for Nucelar Energy in Agriculture (CENA) is a specialized institute of the University of Sao Paulo, located in Piracicaba, Sao Paulo state, in the Agriculture campus.